​

Alanine-scanning of the yeast killer toxin K2 reveals key residues for activity, gain-of-function variants, and supports prediction of precursor processing and 3D structure

Rianne C. Prins, Tycho Marinus, Eyal Dafni, Iftach Yacoby, Sonja Billerbeck  

bioRxiv: doi: https://doi.org/10.1101/2024.11.22.624868 (2024)

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Peptide self-assembly as a strategy for facile immobilization of redox enzymes on carbon electrodes

Tzachi Grinberg, Oren Ben Zvi,  Lihi Adler-Abramovich* and Iftach Yacoby*

Carbon Energy: e411 (2023) 

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A PSII photosynthetic control is activated in anoxic cultures of green algae following illumination

Yuval Milrad, Valéria Nagy, Tamar Elman, Maria Fadeeva, Szilvia Z. Tóth and Iftach Yacoby 

Communications Biology volume 6, Article number: 514 (2023) 

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A two-phase protocol for ambient hydrogen production using Chlamydomonas reinhardtii

Tamar Elman and Iftach Yacoby 

STAR protocols, (2022):3:101640

 

Advances and challenges in photosynthetic hydrogen production 

Kevin E. Redding, Jens Appel, Marko Boehm, Marc M. Nowaczyk, Iftach Yacoby and Kirstin Gutekuns

Published on May 2022

Trends in Biotechnology

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ENHANCED CHLOROPLAST-MITOCHONDRIA CROSSTALK PROMOTES AMBIENT ALGAL-H2 PRODUCTION

Tamar Elman,  Thi Thu Hoai Ho, Yuval Milrad, Michael Hippler and Iftach Yacoby 

Cell Reports Physical Science, (2022): 3(4): 100828

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Photosystem I light-harvesting proteins regulate photosynthetic electron transfer and hydrogen production.   

Thi Thu Hoai Ho, Chris Schwier, Tamar Elman, Vera Fleuter, Karen Zinzius, Martin Scholz, Iftach Yacoby, Felix E Buchert, Michael Hippler *

Published on Dec 2021

Plant Physiology

*Corresponding author

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Use of plant chloroplast RNA-binding proteins as orthogonal activators of chloroplast transgenes in the green alga Chlamydomonas reinhardtii

Noam Shahar, Tamar Elman, Rosalind Williams-Carrier, Oren Ben-Zvi, Iftach Yacoby *,
Alice Barkan*

Published on Oct 2021

Algal Reaserch

*Corresponding authors

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Phylloquinone is the principal Mehler reaction site within photosystem I in high light

Marina Kozuleva, Anastasia Petrova, Yuval Milrad, Alexey Semenov, Boris Ivanov, Kevin E Redding, Iftach Yacoby *

Published on May 2021

Plant Physiology

*Corresponding author

​

Protection of Oxygen-Sensitive Enzymes by Peptide Hydrogel

Oren Ben-Zvi, Itzhak Grinberg, Asuka A Orr, Dror Noy, Phanourios Tamamis, Iftach Yacoby*, Lihi Adler-Abramovich*

Published on April 2021

ACS Nano

*Corresponding authors  

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Bi-directional electron transfer between H2 and NADPH mitigates light fluctuation responses in green algae

Yuval Milrad, Shira Schweitzer, Yael Feldman, Iftach Yacoby*

Published on February 2021

Plant Plant Physiology

*Corresponding author  

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Engineered clostridial [FeFe]-hydrogenase shows improved O2 tolerance in Chlamydomonas reinhardtii

Tamar Elman, Shira Schweitzer,  Noam Shahar, James Schwartz and Iftach Yacoby* 

Published on Aug 2020

International Journal of Hydrogen Energy

*Corresponding author  

​​

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Rewiring photosynthesis: a Photosystem I-hydrogenase chimera that makes H2 in vivo

Andrey Kanygin,   Yuval Milrad,   Chandrasekhar Thummala,   Kiera T. Reifschneider,   Patricia Baker,   Pini Marcu,   Iftach Yacoby*  and  Kevin E Redding*

Published on Apr 2020

Energy and Environmental Science

*Corresponding authors  

​

The Integration of Multiple Nuclear- Encoded Transgenes in the Green Alga Chlamydomonas reinhardtii Results in Higher Transcription Levels

Noam Shahar, Shira Landman, Iddo Weiner, Tamar Elman, Eyal Dafni, Yael Feldman, Tamir Tuller* and Iftach Yacoby*

Published on Feb 2020

Frontiers in Plant Sciences

*Corresponding authors  

 

CSO – A sequence optimization software for engineering chloroplast expression in Chlamydomonas reinhardtii

Iddo Weiner, Yael Feldman, Noam Shahar, Iftach Yacoby* and Tamir Tuller*

Published on Dec 2019

Algal Research

*Corresponding authors  

 

Re-routing photosynthetic energy for continuous hydrogen production in vivo

Oren Ben-Zvi, Eyal Dafni, Yael Feldman, and Iftach Yacoby*

Published on November 2019

Biotechnology for Biofuels

*Corresponding author

​

 

Solving the riddle of the evolution of Shine-Dalgarno based translation in chloroplasts

Iddo Weiner, Noam Shahar, Pini Marco, Iftach Yacoby* and Tamir Tuller*

Published on September 2019

Molecular Biology and Evolution

*Corresponding authors  

 

Binding of ferredoxin NADP+ oxidoreductase (FNR) to plant photosystem I

Pini Marco, Tamar Elman, and Iftach Yacoby*

Published on July 2019

Biochimica et Biophysica Acta

 

Paradigm Shift in Algal H2 Production: Bypassing Competitive Processes

Szilvia Z.Tóth* and Iftach Yacoby*

Published on June 2019

Trends in Biotechnology

*Corresponding authors  

 

Prediction and large-scale analysis of primary operons in plastids reveals unique genetic features in the evolution of chloroplasts

Noam Shahar, Iddo Weiner, Lior Stotsky, Tamir Tuller*, Iftach Yacoby*

Published on March 2019

Nucleic Acids Research

*Correspondence authors

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ChimeraUGEM: unsupervised gene expression modeling in any given organism

Alon Diament, Iddo Weiner, Noam Shahar, Shira Landman, Yael Feldman, Shimshi Atar, Meital Avitan, Shira Schweitzer, Iftach Yacoby*, Tamir Tuller*

Published on February 2019

Bioinformatics

*Correspondence authors

​

Image processing software for high throughput quantification of colony luminescence

Eyal Dafni, Iddo Weiner, Noam Shahar, Tamir Tuller*, Iftach Yacoby*

Published on January 2019

mSphere

*Correspondence authors

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Overcoming the expression barrier of the ferredoxin‑hydrogenase chimera in Chlamydomonas reinhardtii supports a linear increment in photosynthetic hydrogen output

Iddo Weiner, Noam Shahar, Yael Feldman, Shira Landman, Yuval Milrad, Oren Ben-Zvi, Meital Avitan, Eyal Dafni, Shira Schweitzer, Haviva Eilenberg, Shimshi Atar, Alon Diament, Tamir Tuller*, Iftach Yacoby*

Published on July 2018

Algal Research

*Correspondence authors

​

Green algal hydrogenase activity is outcompeted by carbon fixation before inactivation by oxygen takes place

Yuval Milrad, Shira Schweitzer, Yael Feldman, Iftach Yacoby*

Published on May 2018

Plant Physiology

*Correspondence author

​

Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events

Pini Marco, Marina Kozuleva, Haviva Eilenberg, Yuval Mazor, Peter Gimeson, Andrey Kanygin, Kevin Redding, Iddo Weiner and Iftach Yacoby*

Published on April 2018

Biochimica et Biophysica Acta

*Correspondence author

​

Enhancing Heterologous Expression in Chlamydomonas reinhardtii by Transcript Sequence Optimization

Iddo Weiner, Shimshi Atar, Shira Schweitzer, Haviva Eilenberg, Yael Feldman, Meital Avitan, Mor Blau, Avihai Danon, Tamir Tuller*, and Iftach Yacoby*

Published on April 2018

The Plant Journal

*Correspondence authors
 

A myovirus encoding both photosystem I and II proteins enhances cyclic electron flow in infected Prochlorococcus cells

​Svetlana Fridman, José Flores-Uribe, Shirley Larom, Onit Alalouf, Oded Liran, Iftach Yacoby, Faris Salama, Benjamin Bailleul, Fabrice Rappaport, Tamar Ziv, Itai Sharon, Francisco M. Cornejo-Castillo, Alon Philosof, Christopher L. Dupont, Pablo Sánchez, Silvia G. Acinas, Forest L. Rohwer, Debbie Lindell & Oded Béjà

Published on October 2017

Nature Microbiology 

​

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The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance

Eilenberg H, Weiner I, Ben-Zvi O, Pundak C, Marmari A, Liran O, Wecker SM Milrad Y, and Yacoby I*

Published on August 2016

Biotechnology for Biofuels

*Correspondence author

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Microoxic niches within the thylakoid stroma of air-grown C. reinhardtii protect [FeFe]-hydrogenase

Liran O, Semyatich R, Milrad Y, Eilenberg H, weiner I and Yacoby I*

Published on September 2016

Plant Physiology

*Correspondence author

​

The In-vitro enhancement of FeFe Hydrogenase activity By Superoxide Dismutase

Ben-Zvi O and Yacoby I* 

Published on October 2016

International Journal of  Hydrogen Energy

*Correspondence author

 

Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase

Yacoby I*, Tegler L, Pochkailov S, Zhang S*, King P*.  
Published on April 2012

PLoS1 
*Correspondence authors

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Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin: NADP+-oxidoreductase (FNR) enzymes in vitro

Yacoby I*, Pochekailov S, Toporik H, Ghirardi ML, King PW*, Zhang S*. 
Published on May 2011

Proc Natl Acad Sci U S A 

*Correspondence authors